Microsphere Lithography and a Demonstration of Fluorescence Quenching and Reduced Photobleaching

Using microsphere lithography, a bottom-up fabrication method, we create large areas of gold Fischer pattern. Additionally, using a Polydimethylsiloxane (PDMS) substrate coated with a thin layer of gold, we demonstrate fluorescence quenching and enhancement when the PDMSAu sample is in contact with the fluorescent dye Nile Blue. Additionally, reduced photobleaching of the Nile Blue is shown. Observations are made by raster scanning the sample in an inverted confocal microscope with laser excitation.